Microfluidic Electrochemistry Meets Trapped Ion Mobility Spectrometry and High-Resolution Mass Spectrometry—In Situ Generation, Separation, and Detection of Isomeric Conjugates of Paracetamol and Ethoxyquin

Over the last 3 decades, electrochemistry (EC) has been successfully applied in phase I and II metabolism simulation studies. The electrochemically generated metabolite-like oxidation products can react with selected reagents to form conjugates. During conjugate formation, generation of isomeric compounds is possible. Such conjugates are often separated by high-performance liquid chromatography (HPLC). Here, we demonstrate a powerful approach that combines EC ion mobility spectrometry separate possible In detail, present hyphenation microfluidic electrochemical chip an integrated mixer coupled online trapped (TIMS) time-of-flight high-resolution mass (ToF-HRMS), briefly chipEC-TIMS-ToF-HRMS. This novel method achieves results several minutes, which much faster than traditional separation approaches like HPLC, was drug paracetamol controversial feed preservative ethoxyquin. analytes were oxidized situ under formation reactive intermediates mixed different thiol-containing These analyzed TIMS-ToF-HRMS identify isomers. It observed both ethoxyquin two conjugates, characterized mobilities, each reagent. Therefore, using this hyphenated technique, it not only their but also detect these within few minutes.